Materials
| 1000 mL | |
|---|---|
| Warm dd H2O (45°C) | 670 ml |
| MgSO4 • 7 H2O | 10 g |
| Citric acid monohydrate | 100 g |
| K2HPO4 | 500 g |
| NaHNH4PO4 • 4H2O | 175 g |
Procedure
Add salts in the order indicated to warm water in a 2-liter flask placed on magnetic stirring hot plate. Allow each salt to dissolve completely before adding the next. Adjust the volume to 1 liter. Distribute into two 1-liter glass bottles. Autoclave for 20 min at 121°C, store at RT.