Lactobacillus planarum miniprep-PDF

Overview

Method to miniprep plasmid DNA from Lactobacillus plantarum

Procedure

1. Supplement Qiagen P1 buffer with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase.
2. incubate at 37°C for 15-30 minutes.
3. Add Qiagen P2 buffer and continue miniprep as usual.
4. Verify plasmid existence by sequencing or running digests on gel

References

Relevant papers and books

Mathiesen, G., H. M. Namlos, et al. (2004). “Use of bacteriocin promoters for gene expression in Lactobacillus plantarum C11.” Journal of Applied Microbiology 96(4): 819-827.