Overview
General overview and guidelines on how to grow up a culture of Lactobacillus
Materials
- MRS broth (disco)
- MRS agar (disco)
- anaerobic conditions
Procedure
- Follow instructions to make MRS plates and media, autoclave to sterilize
- Streak Lactobacillus on MRS plate or inoculate into a test tube of MRS media
- Incubate at 37C under anaerobic conditions
- If from a new source of Lactobacillus (ex. dehydrated bacteria from ATCC) allow up to 3 days to grow. Otherwise, allow 12-24 hours to grow