LIC Cloning-PDF

Overview

Clone genes into pEU-E01-LIC1 protein expression vector using ligation-independent cloning (LIC).

Materials

List reagents, supplies, and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers’ page on that material are also appropriate.

• supply 1 (i.e. tubes of a certain size? or spreaders?)
• reagent 1
• X μL reagent 2
  • component A (reagent 2 is made up of multiple components)
  • component B
• equipment 1
• equipment 2

Procedure

1. Step 1
2. Step 2
   • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
3. Step 3
   1. Step 3 has multiple sub-steps within it.
   2. Enumerate each of those.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

1. List troubleshooting tips here.
2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding ”’*~~~~”’: to the beginning of your tip.

References

Relevant papers and books

1. Bardóczy V, Géczi V, Sawasaki T, Endo Y, and Mészáros T. A set of ligation-independent in vitro translation vectors for eukaryotic protein production. BMC Biotechnol. 2008 Mar 27;8:32. DOI:10.1186/1472-6750-8-32 | PubMed ID:18371187 | HubMed [Bardoczy-BMCBiotechnol-2008]