Monday, December 23, 2024

zhourui

Pouring LB plates from prepped media-PDF

Notes Melt LB ahead of time because it cools to 55 degrees slowly. A good way to do this is melt it in the...

Nested RT-PCR-PDF

Polymerase dependent expansion of a DNA amplicon Korey Griffin Resource List qPCR Advantages Collect reliable data at low cycle numbers. Traditional PCR...

RNA extraction-PDF

Comparison of purification approaches Guanidine isothiocyanate extraction coupled with lithium chloride precipitation Lyses cells, extracts cellular RNA and denatures proteins all at once in many...

Supplemented M9 broth (M9sup)-PDF

Materials 50 mL 500 mL 1000 mL dH2O 43 ml 430 ml 860 ml 10 X M9 salts + glucose 5 ml 50 ml 100 ml 10% Casamino acids 1 ml 10 ml 20 ml 1% Thiamine 50 µl 0.5 ml 1 ml 1...

Making frozen permanents-PDF

Materials Cryo vials Overnight bacterial culture LB (50% glycerol) Procedure Pipette 400 μL LB 50% glycerol and 600 μL overnight culture into a cryo vial. Vortex,...

Digestion-PDF

Overview Standard DNA single or double digest of variable volume V using NEB restriction enzymes. Materials X μL DNA template 10X NEB Digestion Buffer Y (Y...

Electrocompetent cells-PDF

Materials GYT (glycerol, yeast extract, tryptone) • 10%(v/v) glycerol • 0.125% (w/v) yeast extract • 0.25% (w/v) tryptone DI water 10% Glycerol Special Equipment Centrifuge ...

Chung cells-PDF

Materials Overnight E. coli culture 50 mL Falcon tubes LB TSS 1.5 mL centrifuge tubes Storage: Freezer box Transformation: LB or TSS with 20 mM glucose ...

Join our community of SUBSCRIBERS and be part of the conversation.

To subscribe, simply enter your email address on our website or click the subscribe button below. Don't worry, we respect your privacy and won't spam your inbox. Your information is safe with us.