Monday, December 23, 2024

Diagnostics

Pouring LB plates from prepped media-PDF

Notes Melt LB ahead of time because it cools to 55 degrees slowly. A good way to do this is melt it in the morning then leave it in...

Antigen retrieval-PDF

Detection techniques using antibodies often fail to work on PFA- or formalin-fixed, paraffin-embedded sections. Antigen retrieval methods can then, in some cases, enable specific...

TSA (Tryptone Soy Agar)-PDF

Materials 500 mL: 15 g TSB (Bacto) 500 ml dH2O 1000 mL: 30 g TSB (Bacto) 1000 ml dH2O Procedure Dissolve medium in water. Add 7.5 g Bacto...

TNB (Tryptone Nutrient Broth)-PDF

Materials 500 mL: 2.5 g Tryptone 1.25 g Yeast extract 0.5 g Glucose 4.25 g NaCl 500 ml dH2O Procedure Mix all ingredients in a 1 litre...

LB agar-PDF

Materials 1000 mL: 10 g Bacto tryptone 10 g NaCl 5 g Yeast extract 1000 ml dH2O 7.5 g Bacto agar Procedure Dissolve medium in water. Add...

aureus genomic DNA isolation-PDF

Overview Column-based methods for DNA purification were optimized for E. coli and may not give good yields for other types of bacteria. The following is...

Arabidopsis gDNA isolation-PDF

This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons....

Acetobacter Xylinum Culture-PDF

Overview General guidelines on how to grow a culture of Acetobacter xylinum, ATCC strain 53582. Preparation of Acetobacter Media To prepare ∼500 ml of liquid Acetobacter media,...

Isolate leydig cells from testes-PDF

leydig cell from testes I) Removal of testes: Put into ice-chilled D-PBS. II) Cannulation: Perfuse 0.5 ml DB (1 mg per ml collagenase + one 1...

Rapid plant gDNA extraction-PDF

Abstract This protocol was developed to rapidly obtain genomic DNA from both Arabidopsis thaliana and Medicago truncatula for use in PCR-based analysis. This method has...