Diagnostics
Pouring LB plates from prepped media-PDF
Notes
Melt LB ahead of time because it cools to 55 degrees slowly. A good way to do this is melt it in the morning then leave it in...
Diagnostics
Antigen retrieval-PDF
Detection techniques using antibodies often fail to work on PFA- or formalin-fixed, paraffin-embedded sections. Antigen retrieval methods can then, in some cases, enable specific...
Diagnostics
TSA (Tryptone Soy Agar)-PDF
Materials
500 mL:
15 g TSB (Bacto)
500 ml dH2O
1000 mL:
30 g TSB (Bacto)
1000 ml dH2O
Procedure
Dissolve medium in water. Add 7.5 g Bacto...
Diagnostics
TNB (Tryptone Nutrient Broth)-PDF
Materials
500 mL:
2.5 g Tryptone
1.25 g Yeast extract
0.5 g Glucose
4.25 g NaCl
500 ml dH2O
Procedure
Mix all ingredients in a 1 litre...
Diagnostics
LB agar-PDF
Materials
1000 mL:
10 g Bacto tryptone
10 g NaCl
5 g Yeast extract
1000 ml dH2O
7.5 g Bacto agar
Procedure
Dissolve medium in water. Add...
Diagnostics
aureus genomic DNA isolation-PDF
Overview
Column-based methods for DNA purification were optimized for E. coli and may not give good yields for other types of bacteria. The following is...
Diagnostics
Arabidopsis gDNA isolation-PDF
This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons....
Diagnostics
Acetobacter Xylinum Culture-PDF
Overview
General guidelines on how to grow a culture of Acetobacter xylinum, ATCC strain 53582.
Preparation of Acetobacter Media
To prepare ∼500 ml of liquid Acetobacter media,...
Diagnostics
Isolate leydig cells from testes-PDF
leydig cell from testes
I) Removal of testes: Put into ice-chilled D-PBS.
II) Cannulation: Perfuse 0.5 ml DB (1 mg per ml collagenase + one 1...
Diagnostics
Rapid plant gDNA extraction-PDF
Abstract
This protocol was developed to rapidly obtain genomic DNA from both Arabidopsis thaliana and Medicago truncatula for use in PCR-based analysis. This method has...