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This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons. We only use this with...
Overview
General guidelines on how to grow a culture of Acetobacter xylinum, ATCC strain 53582.
Preparation of Acetobacter Media
To prepare ∼500 ml of liquid Acetobacter media,...
Abstract
This protocol was developed to rapidly obtain genomic DNA from both Arabidopsis thaliana and Medicago truncatula for use in PCR-based analysis. This method has...
Materials
Glass culture tubes with metal caps and labels
Growth medium, from media room or customized
Glass pipette tubes
Parafilm
Equipment
Vortexer
Fireboy or Bunsen...
Polar Lipid Analysis
From DSM Manual, courtesy Hans-Juergen Busse.
Extraction and analysis of respiratory lipoquinones.
A number of methods have been described for the extraction of respiratory...
Plant material:In vitro plants of Nicotiana tabacum L. cv Wisconsin 38
Day 1 Prepare bacteria: • Streak YM plus antibiotic plates with bacteria. • Incubate...
