Monday, December 23, 2024

IVD (In Vitro Diagnostics)

Antigen retrieval-PDF

Detection techniques using antibodies often fail to work on PFA- or formalin-fixed, paraffin-embedded sections. Antigen retrieval methods can then, in some cases, enable specific antibody detection. They work by...

aureus genomic DNA isolation-PDF

Overview Column-based methods for DNA purification were optimized for E. coli and may not give good yields for other types of bacteria. The following is...

Preparing chemically competent cells (Inoue)-S2-PDF

Materials Plate of cells streaked for single colonies SOB Ice TB buffer DMSO Dry Ice (or liquid nitrogen) Glassware & equipment 2 liter Erlenmeyer flask...

Transforming chemically competent cells (Inoue)-S2-PDF

Method Thaw 25 - 200 μl TB buffer cells on ice. Do not use glass tubes, which adsorb DNA. Add DNA, pipette gently to...