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leydig cell from testes
I) Removal of testes: Put into ice-chilled D-PBS.
II) Cannulation: Perfuse 0.5 ml DB (1 mg per ml collagenase + one 1 ml aliquot of 2.4 U/ml...
Overview
In order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the cells can be...
Tamoxifen administration via Chow
pro: less labor-intensive and less stressful for the animals
con: dosage depends on eating behavior; induction may take longer than...
Overview
Staining for intracellular cytokines, followed by flow cytometry analysis, can provide single-cell information about a cell population that one cannot obtain from surface staining...
Material
proteinase K
Taq 10x buffer
tabletop shaker/incubator
Procedure
tissue lysis to release DNA
per tail or tissue chunk add (tissue degradation is sped up if tissue/tail...
