Monday, December 23, 2024

assay development

Supplemented M9 broth (M9sup)-PDF

Materials 50 mL 500 mL 1000 mL dH2O 43 ml 430 ml 860 ml 10 X M9 salts + glucose 5 ml 50 ml 100 ml 10% Casamino acids 1 ml 10 ml 20 ml 1% Thiamine 50 µl 0.5 ml 1 ml 1 M MgSO4 100 µl 1 ml 2 ml 1...

Making frozen permanents-PDF

Materials Cryo vials Overnight bacterial culture LB (50% glycerol) Procedure Pipette 400 μL LB 50% glycerol and 600 μL overnight culture into a cryo vial. Vortex,...

Digestion-PDF

Overview Standard DNA single or double digest of variable volume V using NEB restriction enzymes. Materials X μL DNA template 10X NEB Digestion Buffer Y (Y...

Electrocompetent cells-PDF

Materials GYT (glycerol, yeast extract, tryptone) • 10%(v/v) glycerol • 0.125% (w/v) yeast extract • 0.25% (w/v) tryptone DI water 10% Glycerol Special Equipment Centrifuge ...

Chung cells-PDF

Materials Overnight E. coli culture 50 mL Falcon tubes LB TSS 1.5 mL centrifuge tubes Storage: Freezer box Transformation: LB or TSS with 20 mM glucose ...

E. coli Electroporation-v1-PDF

Overview This a protocol for preparing and handling E. coli cells for electroporation. This protocol has mostly been adapted from the Noyes Lab protocol. A...

S liquid medium-PDF

Overview For Bcc EPS production. Materials 1000 mL: 1 g Yeast extract 10 g Na2HPO4 3 g KH2PO4 1 g K2SO4 1 g NaCl 900 mL Water Procedure Dissolve...

M9 agar-PDF

Materials 500 mL 1000 mL Bacto Agar 7.5 g 15 g dH2O 450 ml 880 ml 10 X M9 salts + glucose 50 ml 100 ml 1 M MgSO4 1 ml 2 ml (2 mM final) 1 M CaCl2 50 µl 0.1...

T-top (0.5% agar)-PDF

Materials 500 mL: 5 g Tryptone 5 g NaCl 500 ml dH2O Bacto agar 1000 mL: 10 g Tryptone 10 g NaCl 1000 ml dH2O Bacto...

10 X M9 salts

Materials 1000 mL: 700 ml dH2O 128 g Na2HPO4 • 7H2O 30 g KH2PO4 5 g NaCl 10 g NH4Cl Procedure Add salts to water, dissolve, adjust...

Tris-HCl-PDF

1M Tris-HCl, pH 7.5 Materials 1000 mL: 121.12 g Tris base 800 mL dH2O HCl Procedure Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add...