Biologics (antibody+ADC)
Biologics (antibody+ADC)
Nested RT-PCR-PDF
Polymerase dependent expansion of a DNA amplicon
Korey Griffin Resource List
qPCR Advantages
Collect reliable data at low cycle numbers. Traditional PCR is less sensitive/ must proceed...
Biologics (antibody+ADC)
RNA extraction-PDF
Comparison of purification approaches
Guanidine isothiocyanate extraction coupled with lithium chloride precipitation
Lyses cells, extracts cellular RNA and denatures proteins all at once in many...
Biologics (antibody+ADC)
10x Lambda diluent (no gelatin)-PDF
Materials
1000 mL:
60.56 g Tris base
58.0 g NaCl
10 g MgSO4
dH2O to 1000 ml
Procedure
Dissolve in 800 ml dH2O, adjust pH to 7.5...
Biologics (antibody+ADC)
Pathway analysis-PDF
After determining a list of genes involved in a given biological process the next step is to map these genes to known pathways/Gene Ontology...
Biologics (antibody+ADC)
Calcium chloride competent cells-PDF
The following protocol is for the preparation of chemically competent E. coli using calcium chloride. It is adapted from Current Protocols in Molecular Biology:
Seidman,...
Biologics (antibody+ADC)
S. aureus pre-treatment for plasmid preps-PDF
Materials
Lysostaphin solution
Overnight S. aureus culture
Plasmid prep kit
Procedure
Lysostaphin stock preparation
1 mg/ml in 20 mM sodium acetate, pH 4.5.
Make up ~50 ml...
Biologics (antibody+ADC)
DNA Microarray Protocol-PDF
Overview
This protocol describes all the steps necessary to perform aRNA synthesis, labeling, hybridization, and scanning of DNA microarrays for Saccharomyces cerevisiae, beginning with total...
Biologics (antibody+ADC)
Miniprep/Kit-free high-throughput protocol-PDF
Background
This protocol is adapted from "Molecular Cloning: A Laboratory Manual", Second Edition, Sambrook, Fritsch, and Maniatis. It is a quick, inexpensive way to purify...
Biologics (antibody+ADC)
Preparing phage specimens for TEM-PDF
Materials
Phage sample
5 mM MgSO4 (or lambda dil)
2% Uranyl acetate in water
Uranyl acetate is highly toxic—wear gloves and safety glasses while handling....
Biologics (antibody+ADC)
Subculturing phage isolates-PDF
Overview
Environmental enrichments or other phage mixtures must be purified to produce pure phage isolates suitable for characterization. Analogous to bacteria, each phage plaque is...
Biologics (antibody+ADC)
Templiphi-PDF
Use this if you want amplify a plasmid that cannot be induced. Can be used to amplify most kinds of circular DNA. The kit...