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Concept
Tissue culture cells need to be "split" to keep them alive. The process involves taking a portion of the cells and resuspending them in fresh culture media so they...
Overview
This short protocol describes how to freeze down and thaw (or bring up) mammalian cells (e.g. HeLa, KEK293, CHO, Jurkat T cells).
Materials
List reagents, supplies,...
Overview
This method is appropriate for purposefully losing an undesired plasmid from your strain of interest or for measuring the approximate rate of plasmid loss...
