Invasive growth assay-PDF

Overview

This is a protocol for how to do yeast invasive growth assay.

Protocol

1. Make YPD plates with 1M Sobitol.
2. Grow strains to saturate.
3. Do serial dilution in 96 well plate.
4. Frog to 1M Sobitol YPD plate.
5. Grow the plate in 30 degree for 2-3 days, then rub the plate under steam of deionized water.
6. Proceed to take picture.