Lactobacillus culture-PDF

Overview

General overview and guidelines on how to grow up a culture of Lactobacillus

Materials

• MRS broth (disco)
• MRS agar (disco)
• anaerobic conditions

Procedure

1. Follow instructions to make MRS plates and media, autoclave to sterilize
2. Streak Lactobacillus on MRS plate or inoculate into a test tube of MRS media
3. Incubate at 37C under anaerobic conditions
4. If from a new source of Lactobacillus (ex. dehydrated bacteria from ATCC) allow up to 3 days to grow. Otherwise, allow 12-24 hours to grow