Materials
1000 mL:
- 10 g Bacto tryptone
- 10 g NaCl
- 5 g Yeast extract
- 1000 ml dH2O
- 7.5 g Bacto agar
Procedure
Dissolve medium in water. Add 7.5 g Bacto agar to empty 1 L bottles, aliquot 500 ml of medium to each bottle. Label caps with “LB” to avoid confusion. Immediately after autoclaving, swirl media to dissolve the melted agar. Tighten caps and place bottles in the water bath. Pour plates when the agar temperature is 50-60 ºC.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!