Materials
| 500 mL | 1000 mL | ||
|---|---|---|---|
| Bacto Agar | 7.5 g | 15 g | |
| dH2O | 430 ml | 860 ml | |
| 10 X M9 salts + glucose | 50 ml | 100 ml | |
| 10% Casamino acids | 10 ml | 20 ml | (0.2Â % final) |
| 1% Thiamine | 0.5 ml | 1 ml | (10 µg/ml final) |
| 1 M MgSO4 | 1 ml | 2 ml | (2 mM final) |
| 1 M CaCl2 | 50 µl | 0.1 ml | (0.1 mM final) |
Procedure
Autoclave agar/water solution, let cool. Aseptically add sterile salts, glucose, casamino acids, and thiamine, pour.