Materials
| 50 mL | 500 mL | 1000 mL | |
|---|---|---|---|
| dH2O | 43 ml | 430 ml | 860 ml |
| 10 X M9 salts + glucose | 5 ml | 50 ml | 100 ml |
| 10% Casamino acids | 1 ml | 10 ml | 20 ml |
| 1% Thiamine | 50 µl | 0.5 ml | 1 ml |
| 1 M MgSO4 | 100 µl | 1 ml | 2 ml |
| 1 M CaCl2 | 5 µl | 50 µl | 0.1 ml |
Procedure
Pre-sterilize all component solutions and aseptically add to sterile water. Alternatively, mix all components and then filter-sterilize through a 0.22 µm filter. Store at 4 ºC.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!