Tag:
Bacterial Culture
Diagnostics
Bacterial cell culture-PDF
Materials
Glass culture tubes with metal caps and labels
Growth medium, from media room or customized
Glass pipette tubes
Parafilm
Equipment
Vortexer
Fireboy or Bunsen...
cell therapy
Plasmid Loss Assay-PDF
Overview
This method is appropriate for purposefully losing an undesired plasmid from your strain of interest or for measuring the approximate rate of plasmid loss...
assay development
Preparing electrocompetent cells-PDF
Materials
Equipment
-80°C freezer
37°C incubator
Refrigerated centrifuge that accepts 225 mL culture tubes
Chemicals and reagents
~500 mL LB Lennox supplemented with appropriate concentration of...
assay development
Lactobacillus transformation-PDF
Overview
This is basically a combination of the version of the Berthier (1996) protocol and the Mason (2005) protocol. This protocol is designed for use...
assay development
Electroporation of E. coli-PDF
Procedure
1. Chill the electroporation cuvettes by floating them in an ice bath.
2. Remove vials containing 100μl electro-competent cells from the -80°C freezer and thaw...