Monday, December 23, 2024
Tag:

bacterial transformation

Preparing chemically competent cells-S2-PDF

Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical...

DNA Spots-PDF

Materials For making Spots: 1% Cresol Red DNA (100 ng/μL) Crane & Co. 100% cotton, acid free thesis paper For using spots to transform: ...

Transform by Electroporation-PDF

Materials 50mL 7H9 mycobacterial medium + 3mL per transformation 102mL 10% glycerol (possibly a few mL more if you are doing many transformations) 400mL...

High Efficiency Transformation-PDF

Protocol Day 0 Make sure you have the necessary solutions (instructions for how to make them can be found here): Single-stranded carrier DNA PEG 3350...