Tag:
bacterial transformation
assay development
Preparing chemically competent cells-S2-PDF
Materials
Plate of cells to be made competent
TSS buffer
LB media
Ice
Glassware & Equipment
Falcon tubes
500μl Eppendorf tubes, on ice
200ml conical...
assay development
DNA Spots-PDF
Materials
For making Spots:
1% Cresol Red
DNA (100 ng/μL)
Crane & Co. 100% cotton, acid free thesis paper
For using spots to transform:
...
assay development
Transform by Electroporation-PDF
Materials
50mL 7H9 mycobacterial medium + 3mL per transformation
102mL 10% glycerol (possibly a few mL more if you are doing many transformations)
400mL...
Biologics (antibody+ADC)
High Efficiency Transformation-PDF
Protocol
Day 0
Make sure you have the necessary solutions (instructions for how to make them can be found here):
Single-stranded carrier DNA
PEG 3350...