Monday, December 23, 2024
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Cell Culture

Agarose Slides for Imaging-PDF

Overview This protocol allows live bacterial cell imaging. We create a small, nutrient rich gel pad and inject the bacteria on top. Materials 500mg Agarose 100...

Preparing electrocompetent cells-PDF

Procedure Liquid Nitrogen must be purchased from the Chemistry-Stockroom by 11:30AM. Day 1 1. Autoclave the following 9x125ml flasks containing 50 mL LB Lennox or SOB ...

Mouse keratinocyte cultures-PDF

PRIMARY MOUSE KERATINOCYTE CULTURES Isolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al., Methods Enzymol. 254:3-20 (1995). The...

Isolation of murine splenocytes-PDF

Overview In order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the cells can be...