Tag:
Cell Culture
assay development
Agarose Slides for Imaging-PDF
Overview
This protocol allows live bacterial cell imaging. We create a small, nutrient rich gel pad and inject the bacteria on top.
Materials
500mg Agarose
100...
assay development
Preparing electrocompetent cells-PDF
Procedure
Liquid Nitrogen must be purchased from the Chemistry-Stockroom by 11:30AM.
Day 1
1. Autoclave the following
9x125ml flasks containing 50 mL LB Lennox or SOB
...
Biologics (antibody+ADC)
Mouse keratinocyte cultures-PDF
PRIMARY MOUSE KERATINOCYTE CULTURES
Isolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al., Methods Enzymol. 254:3-20 (1995). The...
Diagnostics
Isolation of murine splenocytes-PDF
Overview
In order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the cells can be...