Tag:
Cloning
assay development
Amplified insert assembly-PDF
Overview
This is a lab specific protocol. For more information on the benefits of Amplified Insert Assembly see the consensus protocol. A You-Tube video summary...
gene therapy & gene editing
Making a multi-part plasmid-V1-S2-PDF
Overview
This protocol describes how to assemble multiple-part plasmids via BsaI digestion and T7 Ligase ligation. It is intended to supplement Lee, M. E., DeLoache,...