Tag:
colony PCR
assay development
Rapid bacterial DNA prep-PDF
Low-yield DNA is suitable for PCR.
Materials
Sterile needles or toothpicks
Sterile microtubes
Lysis buffer (in ddH2O): 0.25% (w/v) SDS, 50 mM NaOH; do not...
Biologics (antibody+ADC)
Colony PCR-PDF
Zymolyase Solution:
Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store...