Tag:
DNA Analysis
assay development
Agarose gel electrophoresis-S2-PDF
Casting agarose gels
We precast our gels.
Materials
1X TAE
SYBR safe (10,000X stock)
Agarose
Microwave
Stir plate and stir bar (optional)
Procedure
Add 300mL 1X TAE...
assay development
Agarose Gel Electrophoresis-PDF
Procedure
Place the gel tray perpendicular in the electrophoresis chamber to create a casting bay.
Wipe the tray clean with a Kim-wipe and level...
Diagnostics
Mouse tissue lysis for genotyping-PDF
Material
proteinase K
Taq 10x buffer
tabletop shaker/incubator
Procedure
tissue lysis to release DNA
per tail or tissue chunk add (tissue degradation is sped up if tissue/tail...