Tag:
DNA Ligation
assay development
DNA ligation using NEB Quick Ligation Kit-PDF
Materials
We use the Quick Ligation Kit from NEB
Deionized, sterile H2O
Purified, linearized vector (in H2O)
Purified, linearized insert (in H2O)
Ligation Mix
This is...
assay development
TOPO TA cloning-PDF
Purpose
Efficient cloning of PCR products.
Materials
PCR product generated with Taq polymerase (for 3' A tails)
TOPO cloning kit
Procedure
Reshma's procedure
(Note that the kit details a...
assay development
DNA ligation using T4 DNA ligase-PDF
Materials
We use the T4 DNA ligase from NEB
Deionized, sterile H2O
Purified, linearized vector (in H2O)
Purified, linearized insert (in H2O)
Ligation Mix
X...