Tuesday, December 24, 2024
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DNA Ligation

DNA ligation using NEB Quick Ligation Kit-PDF

Materials We use the Quick Ligation Kit from NEB Deionized, sterile H2O Purified, linearized vector (in H2O) Purified, linearized insert (in H2O) Ligation Mix This is...

TOPO TA cloning-PDF

Purpose Efficient cloning of PCR products. Materials PCR product generated with Taq polymerase (for 3' A tails) TOPO cloning kit Procedure Reshma's procedure (Note that the kit details a...

DNA ligation using T4 DNA ligase-PDF

Materials We use the T4 DNA ligase from NEB Deionized, sterile H2O Purified, linearized vector (in H2O) Purified, linearized insert (in H2O) Ligation Mix X...