Tag:
DNA Sequencing
assay development
RAD-seq-PDF
Overview
This is a protocol for generating RAD libraries for Illumina sequencing. With this technique, 96 samples can be multiplexed into one sequencing library, and...
assay development
Miniprep low copy plasmids-PDF
Purpose
When sequencing miniprepped DNA of low copy plasmids, I see a pretty high sequence failure rate despite my submitting what I think is sufficient...
assay development
Mesoplasma florum:Inverse PCR Transposon location-PDF
Choice of Enzyme
Hutchison used DraI as a cutter, but this is a blunt cutter, making religation difficult
MboI cuts at GATC sites, and...