Tag:
E. coli
assay development
ChIP-Chip E. coli-PDF
Abstract
ChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genome-wide binding sites of...
assay development
DNA Spots-PDF
Materials
For making Spots:
1% Cresol Red
DNA (100 ng/μL)
Crane & Co. 100% cotton, acid free thesis paper
For using spots to transform:
...
assay development
Miniprep low copy plasmids-PDF
Purpose
When sequencing miniprepped DNA of low copy plasmids, I see a pretty high sequence failure rate despite my submitting what I think is sufficient...
assay development
Preparing electrocompetent cells-PDF
Procedure
Liquid Nitrogen must be purchased from the Chemistry-Stockroom by 11:30AM.
Day 1
1. Autoclave the following
9x125ml flasks containing 50 mL LB Lennox or SOB
...
assay development
DNA Miniprep with Alkaline Lysis-PDF
Overview
Miniprep DNA from E. Coli
Materials
STE
Stock
From Stock(for 50ml)
Final
1M Tris(pH8)
0.5ml
10mM
5M NaCl
1ml
100mM
0.5M EDTA
0.1ml
1mM
ALKALINE LYSIS 1
Stock
From Stock(for 50ml)
Final
1M Tris(pH8)
1ml
20mM
Glucose FW:108.2
0.45g
50mM
0.5M EDTA
0.1ml
10mM
ALKALINE LYSIS 2
Stock
From Stock(for 10ml)
Final
5N...