Monday, December 23, 2024
Tag:

E. coli

ChIP-Chip E. coli-PDF

Abstract ChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genome-wide binding sites of...

DNA Spots-PDF

Materials For making Spots: 1% Cresol Red DNA (100 ng/μL) Crane & Co. 100% cotton, acid free thesis paper For using spots to transform: ...

Miniprep low copy plasmids-PDF

Purpose When sequencing miniprepped DNA of low copy plasmids, I see a pretty high sequence failure rate despite my submitting what I think is sufficient...

Preparing electrocompetent cells-PDF

Procedure Liquid Nitrogen must be purchased from the Chemistry-Stockroom by 11:30AM. Day 1 1. Autoclave the following 9x125ml flasks containing 50 mL LB Lennox or SOB ...

DNA Miniprep with Alkaline Lysis-PDF

Overview Miniprep DNA from E. Coli Materials STE Stock From Stock(for 50ml) Final 1M Tris(pH8) 0.5ml 10mM 5M NaCl 1ml 100mM 0.5M EDTA 0.1ml 1mM   ALKALINE LYSIS 1 Stock From Stock(for 50ml) Final 1M Tris(pH8) 1ml 20mM Glucose FW:108.2 0.45g 50mM 0.5M EDTA 0.1ml 10mM   ALKALINE LYSIS 2 Stock From Stock(for 10ml) Final 5N...