Tag:
Electroporation
assay development
Mesoplasma florum:Electroporation-PDF
Materials
overnight culture of Mesoplasma florum in ATCC 1161 medium
preparing 10 ml cultures with 1, 10, 100, and 1000 μl of infected cultures...
assay development
Transform by Electroporation-PDF
Materials
50mL 7H9 mycobacterial medium + 3mL per transformation
102mL 10% glycerol (possibly a few mL more if you are doing many transformations)
400mL...
assay development
Electroporation of E. coli-PDF
Procedure
1. Chill the electroporation cuvettes by floating them in an ice bath.
2. Remove vials containing 100μl electro-competent cells from the -80°C freezer and thaw...