Monday, December 23, 2024
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Electroporation

Mesoplasma florum:Electroporation-PDF

Materials overnight culture of Mesoplasma florum in ATCC 1161 medium preparing 10 ml cultures with 1, 10, 100, and 1000 μl of infected cultures...

Transform by Electroporation-PDF

Materials 50mL 7H9 mycobacterial medium + 3mL per transformation 102mL 10% glycerol (possibly a few mL more if you are doing many transformations) 400mL...

Electroporation of E. coli-PDF

Procedure 1. Chill the electroporation cuvettes by floating them in an ice bath. 2. Remove vials containing 100μl electro-competent cells from the -80°C freezer and thaw...