Tag:
Griffin
assay development
Ultimate Immunoprecipitation Guide-PDF
Cell Culture volumetric parameters
Serum levels of human Ig isotypes
Protein G Binding Capacity
Protein A Binding Capacity
When performing an IP experiment, consider...
assay development
Membrane Stripping and Reprobing-PDF
Tris/2-Me/SDS
The key to a successful stripping and reprobing is to rinse the membrane until there is no beta-mercaptoethanol odor present. This will require several...
assay development
Isolate Leukocytes from whole blood-PDF
Isolate leukocytes from whole blood
Complement Pathway
1) For each 5 mL of blood, add 45 mL of room temperature 0.17 M Ammonium Chloride solution...
Biologics (antibody+ADC)
Dual Immunofluorescence Cell Staining-PDF
Dual Immunofluorescence Labeling Protocol
Dual labeling assay using NUNC Labtek II chamberslide system (Fisher Scientific). For this assay, system was HA epitope-tagged (N-terminal) human P2Y2...
Biologics (antibody+ADC)
Puromycin Selection-PDF
Puromycin dihydrochloride
Puromycin dihydrochloride is a aminonuclease antibiotic that inhibits protein synthesis. Puromycin is used for selection and maintenance of cell lines expressing a transfected...
gene therapy & gene editing
shRNA Transfection-PDF
Lentivirus, shRNA plasmid, or siRNA?
Choosing between Lentivirus (somewhat stable), shRNA transfer vector (stable/transient), or siRNA (transient). Perform and exhaustive literature search (pubmed) for citations...