Tag:
Knight
assay development
Centrifuge desalting/Zeba columns-PDF
Overview
This protocol is for doing buffer exchange on small sample volumes (30-130 μL) volumes.
Materials
Zeba Desalt Spin Columns, 0.5 ml from Pierce
Variable-speed bench-top...
Biologics (antibody+ADC)
NuPAGE electrophoresis-PDF
Purpose
To run a denaturing protein gel. Note that a preferable version of this protocol is available at Sauer:bis-Tris SDS-PAGE, the very best (recommended by...
Biologics (antibody+ADC)
Protein solubility-PDF
Overview
This is a quick protocol to assess how soluble a particular protein is.
Materials
Denaturing lysis buffer
Native lysis buffer
Triton X-100
2 mL centrifuge...
assay development
NuPAGE electrophoresis/Hybrid staining-PDF
Overview
A fast protocol for visualizing bands on a polyacrylamide gel via coomassie-like staining.
Materials
Deionized water
Invitrogen SimplyBlue SafeStain
Microwave
Plastic tray
Use the lid...
assay development
NuPAGE electrophoresis/Gel drying-PDF
Overview
A procedure for drying and preserving a polyacrylamide gel.
Materials
Gel drying solution
20% ethanol
10% glycerol
Gel drying frames from Diversified Biotech
Cellophane sheets...
assay development
Protocols/p11 resin preparation-PDF
Overview
Preparation of Whatmann P11 Phosphocellulose resin for cation exchange chromatography.
Phosphocellulose remains a useful and cheap cation exchange resin and is particularly good for the...