Monday, December 23, 2024
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Molecular Biology

Annealing Oligos-PDF

Overview This is a protocol for annealing oligos for yeast transformation. Materials Forward Oligo Reverse Oligo 10X Annealing Buffer 1X TE buffer Stock Solutions 10X annealing buffer Recipe...

Rapid plant gDNA extraction-PDF

Abstract This protocol was developed to rapidly obtain genomic DNA from both Arabidopsis thaliana and Medicago truncatula for use in PCR-based analysis. This method has...

PCR-PDF

Primer design for PCR General Design considerations. Make sure that: The primer length is between 15-30 bp. I suggest starting with 20-25 bp primers. ...

Annealing and primer extension with Klenow polymerase-PDF

Overview This protocol uses annealing and primer extension to generate a short DNA fragment (~100 bp). The DNA fragment is prepared for cloning by restriction...

Sequencing BioBrick DNA-PDF

Purpose To confirm the physical DNA resulting from a BioBrick standard assembly step has the same sequence as that part in the registry, the physical...

Cloning Checklist-PDF

E. Coli Cloning Checklist This is a rough guide to the DOs and DO NOTs of cloning in E. Coli. It was assembled based on...

Size selective DNA precipitation-PDF

Curators Kersten S. Rabe Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator...

PNK Treatment of DNA Ends-PDF

Introduction This protocol is used to add a phosphate group to the 5' end of a single or double stranded DNA molecule. Most primers, for...