Tag:
Molecular Biology
assay development
Annealing Oligos-PDF
Overview
This is a protocol for annealing oligos for yeast transformation.
Materials
Forward Oligo
Reverse Oligo
10X Annealing Buffer
1X TE buffer
Stock Solutions
10X annealing buffer
Recipe...
Diagnostics
Rapid plant gDNA extraction-PDF
Abstract
This protocol was developed to rapidly obtain genomic DNA from both Arabidopsis thaliana and Medicago truncatula for use in PCR-based analysis. This method has...
assay development
PCR-PDF
Primer design for PCR
General Design considerations. Make sure that:
The primer length is between 15-30 bp. I suggest starting with 20-25 bp primers.
...
assay development
Annealing and primer extension with Klenow polymerase-PDF
Overview
This protocol uses annealing and primer extension to generate a short DNA fragment (~100 bp). The DNA fragment is prepared for cloning by restriction...
assay development
Sequencing BioBrick DNA-PDF
Purpose
To confirm the physical DNA resulting from a BioBrick standard assembly step has the same sequence as that part in the registry, the physical...
Biologics (antibody+ADC)
Cloning Checklist-PDF
E. Coli Cloning Checklist
This is a rough guide to the DOs and DO NOTs of cloning in E. Coli. It was assembled based on...
assay development
Size selective DNA precipitation-PDF
Curators
Kersten S. Rabe
Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator...
gene therapy & gene editing
PNK Treatment of DNA Ends-PDF
Introduction
This protocol is used to add a phosphate group to the 5' end of a single or double stranded DNA molecule. Most primers, for...