Tuesday, December 24, 2024
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Molecular Biology Techniques

Streptavidin purification of DNA fragments-PDF

Biotin Primers Make primers for PCR reactions with a 5' biotin modification HPLC purification of these primers is desirable to eliminate short primers and...

Affymetrix DNA labelling for gene expression arrays-PDF

Affymetrix DNA labelling for gene expression arrays This protocol will allow you to perform the labeling of DNA ready for Affymetrix expression GeneChip analysis. Workflow Materials BioPrime...

Mesoplasma florum:RNA Purification-PDF

Cell Lysis Start with 1 to 2 ml liquid culture. Add 2 volumes RNAprotect Bacteria Reagent. Mix immediately by vortexing for 5 seconds. Incubate...

Phage genomic DNA extraction-PDF

Overview n this protocol you extract the genomic DNA from the phages in a lysate. The lysates are “dirty” in that they contain spent media...

Yeast DNA Prep-PDF

Protocol grow up yeast culture to appropriate density (near saturation) spin 1.5 ml of culture for 1 min in microfuge and aspirate off supernatant ...

High Efficiency Transformation-PDF

Protocol Day 0 Make sure you have the necessary solutions (instructions for how to make them can be found here): Single-stranded carrier DNA PEG 3350...

Colony PCR-PDF

Zymolyase Solution: Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store...