Tag:
Molecular Biology Techniques
assay development
Streptavidin purification of DNA fragments-PDF
Biotin Primers
Make primers for PCR reactions with a 5' biotin modification
HPLC purification of these primers is desirable to eliminate short primers and...
assay development
Affymetrix DNA labelling for gene expression arrays-PDF
Affymetrix DNA labelling for gene expression arrays
This protocol will allow you to perform the labeling of DNA ready for Affymetrix expression GeneChip analysis.
Workflow
Materials
BioPrime...
assay development
Mesoplasma florum:RNA Purification-PDF
Cell Lysis
Start with 1 to 2 ml liquid culture.
Add 2 volumes RNAprotect Bacteria Reagent. Mix immediately by vortexing for 5 seconds.
Incubate...
Biologics (antibody+ADC)
Phage genomic DNA extraction-PDF
Overview
n this protocol you extract the genomic DNA from the phages in a lysate. The lysates are “dirty” in that they contain spent media...
Biologics (antibody+ADC)
Yeast DNA Prep-PDF
Protocol
grow up yeast culture to appropriate density (near saturation)
spin 1.5 ml of culture for 1 min in microfuge and aspirate off supernatant
...
Biologics (antibody+ADC)
High Efficiency Transformation-PDF
Protocol
Day 0
Make sure you have the necessary solutions (instructions for how to make them can be found here):
Single-stranded carrier DNA
PEG 3350...
Biologics (antibody+ADC)
Colony PCR-PDF
Zymolyase Solution:
Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store...