Tag:
plasmid DNA
Biologics (antibody+ADC)
Lactobacillus planarum miniprep-PDF
Overview
Method to miniprep plasmid DNA from Lactobacillus plantarum
Procedure
Supplement Qiagen P1 buffer with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase.
incubate at 37°C for 15-30...
assay development
Transform by Electroporation-PDF
Materials
50mL 7H9 mycobacterial medium + 3mL per transformation
102mL 10% glycerol (possibly a few mL more if you are doing many transformations)
400mL...