Tuesday, December 24, 2024
Tag:

plasmid DNA

Lactobacillus planarum miniprep-PDF

Overview Method to miniprep plasmid DNA from Lactobacillus plantarum Procedure Supplement Qiagen P1 buffer with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase. incubate at 37°C for 15-30...

Transform by Electroporation-PDF

Materials 50mL 7H9 mycobacterial medium + 3mL per transformation 102mL 10% glycerol (possibly a few mL more if you are doing many transformations) 400mL...