Tag:
Yeast
assay development
McClean: Alpha Factor Stock-PDF
Overview
This is the standard 1mg/ml stock solution we use for inducing the pheromone pathway in budding yeast. Generally, we find that the different batches...
assay development
McClean: Colony PCR (Yeast)-PDF
Overview
Our lab's version of yeast colony PCR, was adapted from the Botstein Lab's protocol. Generally, we use this protocol for checking transformations (ie, to...
assay development
McClean: Glycerol stocks (yeast)-PDF
Overview
Yeast strains can be maintained almost indefinitely at -80°C in glycerol stocks. You need to make sure that as soon as you have a...