Tuesday, December 24, 2024
Tag:

Yeast

McClean: Alpha Factor Stock-PDF

Overview This is the standard 1mg/ml stock solution we use for inducing the pheromone pathway in budding yeast. Generally, we find that the different batches...

McClean: Colony PCR (Yeast)-PDF

Overview Our lab's version of yeast colony PCR, was adapted from the Botstein Lab's protocol. Generally, we use this protocol for checking transformations (ie, to...

McClean: Glycerol stocks (yeast)-PDF

Overview Yeast strains can be maintained almost indefinitely at -80°C in glycerol stocks. You need to make sure that as soon as you have a...